Annular‐ring slot antenna designs with circular polarization radiation

The design of a microstrip‐fed annular‐ring slot antenna (ARSA) with circular polarization (CP) radiation is initially studied. To obtain CP radiation with broad 3‐dB axial ratio (AR) bandwidth that can cover the WiMAX 2.3 GHz (2305–2320 MHz, 2345–2360 MHz) and WLAN 2.4 GHz (2400–2480 MHz) bands, a novel technique of extending an inverted L‐shaped slot from the bottom section of the annular‐ring is proposed. To suppress the harmonic modes induced by the CP ARSA, the technique of integrating a defected ground structure into the annular‐ring slot is further introduced. From the measured results, 10‐dB impedance bandwidth and 3‐dB AR bandwidth of 44.86 and 9.68% were achieved by the proposed harmonic suppressed CP ARSA. Furthermore, average gain and radiation efficiency of ～4.7 dBic and 71%, respectively, were also exhibited across the bands of interest. © 2014 Wiley Periodicals, Inc. Int J RF and Microwave CAE 25:337–345, 2015.     

转子/密封系统失稳振动抑制的动力吸振方法研究

针对密封中流体引起转子/密封系统振动失稳的问题。提出采用动力吸振器(DVA)实现转子/密封系统失稳振动抑制。建立转子/密封-DVA系统非线性微分方程,采用数值方法求解并获得附加DVA前后转子/密封系统的非线性特性;根据Hurwitz判据获得系统的临界稳定条件;通过遗传算法优化DVA参数,获得附加优化DVA前后转子/密封系统稳定性。结果表明:附加DVA能够改变转子/密封系统失稳振动频率和失稳阈值;在一定的转速范围内失稳振动被完全抑制;在不完全抑制的转速范围内,附加DVA能降低失稳振动的振幅。     

一种低信噪比环境下的语音端点检测算法

端点检测技术是语音信号处理的关键技术之一，为提高低信噪比环境下端点检测的准确率和稳健性，提出了一种非平稳噪声抑制和调制域谱减结合功率归一化倒谱距离的端点检测算法。该算法首先通过抑制非平稳噪声再采用调制域谱减消除残余噪声来提升信噪比，减少语音失真。然后再提取每帧信号的功率归一化倒谱系数，计算每帧信号与背景噪声的功率归一化倒谱距离。最后将该倒谱距离作为检测参数，采用双门限判决方法进行端点检测。实验结果表明，该端点检测算法对语音帧和噪声帧具有较好的区分性。此外，在低信噪比环境下，所提出的算法对于不同类型的噪声都具有较好的稳健性。     

基于哈希锁定的网络传输异常序列监测仿真

为有效提升传统网络传输异常序列监测方法的处理效率,提出基于哈希锁定的网络传输异常序列监测方法。利用随机投影的哈希函数将高维空间数据转换为低维空间数据,并利用网络传输序列的多元数量值函数,组建网络传输结构模型。对网络传输结构模型进行流量时频采样以及时间序列分析,结合FIR滤波器对其进行流量抗干扰处理,通过高阶累计的振荡衰减,对输出的滤波数据进行特征搜索及提取,实现基于哈希锁定的网络传输异常序列监测。仿真结果表明,所提方法具有较强的抗干扰性,并且能够确保网络的稳定运行。     

改型Wollaston棱镜干涉光谱仪的调制度研究

改型Wollaston棱镜(MWP)无需中继,可构建微型傅里叶变换光谱仪。回顾了MWP的基本原理,从调制度定义出发,计算得到准单色光源的复相干度,找到调制度的影响因素——扩展视场、剪切结构、焦面探测等。建立一般的干涉定域分析模型后,由像点逆光路追迹给出MWP的厚度计算公式,代入调制度约束参数得到不同参量的误差容限。通过FRED记录不同视场扩展情况的灰度与辐照度,结果显示,调制度为0.9时,视场与理论估算值1.24°基本相符;不同剪切量的灰度与辐照度变化特性表明,结构角会使定域垂直系统光轴的入射角发生变化,与晶轴倾角共同影响双折射率差;2.5和8μm像元探测采样表现出调制度差异,描述了定域深度或离焦的影响。以准单色光源、复相干度表示调制度,可将不同影响因素统一至约束参数中,相关变量误差容限可作为MWP-FTS的设计依据。     

Damage propagation and strength prediction of a single-lap interference-fit laminate structure

Frontiers of Mechanical Engineering - Experimental and finite element research was conducted on the bolted interference fit of a single-lap laminated structure to reveal the damage propagation...     

Synthesis of KUE-siRNA Conjugates for Prostate Cancer Cell-Targeted Gene Silencing

The delivery of siRNAs to selectively target cells poses a great challenge in RNAi-based cancer therapy. The lack of suitable cell-targeting methods seriously restricts the advance in delivering siRNAs to extrahepatic tissues. Based on prostate-specific membrane antigen (PSMA)-targeting ligands, we have synthesized a series of lysine-urea-glutamate (KUE)-siRNA conjugates and verified their effective cell uptake and gene silencing properties in prostate cancers. The results indicated that the KUE-siRNA conjugates could selectively enter PSMA⁺ LNCaP cells, eventually down-regulating STAT3 expression. Based on post-synthesis modification and receptor-mediated endocytosis, this strategy of constructing ligand-siRNA conjugates might provide a general method of siRNA delivery for cell-targeted gene silencing.     

Hepatitis B Virus-Like Particle: Targeted Delivery of Plasmid Expressing Short Hairpin RNA for Silencing the Bcl-2 Gene in Cervical Cancer Cells

Gene therapy research has advanced to clinical trials, but it is hampered by unstable nucleic acids packaged inside carriers and there is a lack of specificity towards targeted sites in the body. This study aims to address gene therapy limitations by encapsidating a plasmid synthesizing a short hairpin RNA (shRNA) that targets the anti-apoptotic Bcl-2 gene using truncated hepatitis B core antigen (tHBcAg) virus-like particle (VLP). A shRNA sequence targeting anti-apoptotic Bcl-2 was synthesized and cloned into the pSilencer 2.0-U6 vector. The recombinant plasmid, namely PshRNA, was encapsidated inside tHBcAg VLP and conjugated with folic acid (FA) to produce FA-tHBcAg-PshRNA VLP. Electron microscopy revealed that the FA-tHBcAg-PshRNA VLP has an icosahedral structure that is similar to the unmodified tHBcAg VLP. Delivery of FA-tHBcAg-PshRNA VLP into HeLa cells overexpressing the folate receptor significantly downregulated the expression of anti-apoptotic Bcl-2 at 48 and 72 h post-transfection. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay demonstrated that the cells’ viability was significantly reduced from 89.46% at 24 h to 64.52% and 60.63%, respectively, at 48 and 72 h post-transfection. As a conclusion, tHBcAg VLP can be used as a carrier for a receptor-mediated targeted delivery of a therapeutic plasmid encoding shRNA for gene silencing in cancer cells.